Figure 3.

When the mice were induced by STZ, miR‐382 is upregulated while the FoxO1 is reciprocal, and extracellular matrix proteins are increased in renal cortices of DN mice. Panel A, qRT‐PCR assay was used to determine the expression of miR‐382 and the mRNA levels of FoxO1, FN, Col I, Col IV and PAI‐1; Panels B and C, western blot analysis was used to determine the protein expressions of FoxO1, p‐FoxO1, FN, Col I, Col IV and PAI‐1; the protein expression of FoxO1 does not differ after STZ inducement. *P < .05 vs. the normal mice; 15 normal mice and 30 DN mice; the data are presented as mean ± standard deviation, analysed by Student t test; the experiment was independently repeated three times