Figure 4.

FoxO1 is a target gene of miR‐382 by the target prediction program and determination of luciferase activity. Panel A, miR‐382 binds to the 3′UTR of FoxO1 using the target prediction program, microRNA; Panel B, the luciferase activity is decreased after treatment by a combination of miR‐382 mimics and FoxO1‐3′UTR‐wt, suggesting that miR‐129‐5p regulates FoxO1. In Panel B, the data are presented as mean ± standard deviation, analysed by one‐way ANOVA. The experiment was independently repeated three times. *P < .05, vs. the mimic NC