Figure 5.

Upregulation of miR‐382 negatively regulates FoxO1 and increases ECM accumulation in the glomerular mesangial cells. Panel A, qRT‐PCR assay was used to determine the expression of miR‐382 and the mRNA levels of FoxO1, FN, Col I, Col IV and PAI‐1 in the glomerular mesangial cells; Panels B and C, western blot analysis was used to determine the protein expressions of FoxO1, p‐FoxO1, FN, Col I, Col IV and PAI‐1; the protein expression of FoxO1 does not differ among the six groups. *P < .05 vs. the blank and NC groups. The data are presented as mean ± standard deviation, analysed by Student t test. The experiment was independently repeated three times