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. 2018 Apr 27;51(5):e12462. doi: 10.1111/cpr.12462

Figure 7.

Figure 7

Upregulation of miR‐382 or glomerular FoxO1 affects cell cycle distribution while inhibiting apoptosis in glomerular mesangial cells. Panel A, the DNA content of PI‐stained cells at the G0/G1, S and G2/M phases; Panel B, the percentage of PI‐stained cells at the G0/G1, S, and G2/M phases; Panel C, glomerular mesangial cells in the scatter plots in which the upper left quadrant identifies the necrotic cells (annexin V‐/PI+), the upper right quadrant identifies the late apoptotic cells (annexin V+/PI+), the lower left quadrant identifies the live cells (annexin V‐/PI‐) and the lower right quadrant identifies the early apoptotic cells (annexin V+/PI‐). Panel D, the percentage of early and late apoptotic cells. *P < .05 vs the blank and NC groups. The data are presented as mean ± standard deviation, analysed by a one‐way ANOVA. The experiment was independently repeated three times