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. 2017 Nov 30;51(1):e12409. doi: 10.1111/cpr.12409

Figure 2.

Figure 2

Cancer susceptibility candidate 2 (CASC2) suppressed the osteosarcoma cell proliferation. A, The expression of CASC2 in the osteosarcoma cell lines (MG‐63, U2OS, SOSP‐9607 and Saos‐2) and human osteoblasts cell line (hFOB) was measured by qRT‐PCR. U6 was used as the internal control. B, The expression level of CASC2 in the MG‐63 cell with transfecting pcDNA‐CASC2 vector was determined using qRT‐PCR. C, The expression level of CASC2 in the U2OS cell was measured by qRT‐PCR. D, Ectopic expression of CASC2 decreased the MG‐63 proliferation. The cell proliferation was determined by CCK‐8 assay. E, CCK‐8 assay was performed to measure the U2OS cell proliferation. *P < .05, **P < .01 and ***P < .001