Figure 5.

Ras association domain family member 6 (RASSF6) expression was downregulated in the osteosarcoma tissues. A, The RASSF6 expression in the osteosarcoma samples and non‐tumour tissues using qRT‐PCR. GAPDH was used as the control. B, The RASSF6 expression was significantly downregulated in the osteosarcoma tissues compared with non‐tumorous samples. C, The lower expression of RASSF6 was associated with the advanced TNM stage. D, The expression of RASSF6 was positively correlated with the expression of CASC2 in the osteosarcoma tissues. E, The expression level of RASSF6 in the osteosarcoma cell lines (MG‐63, U2OS, SOSP‐9607 and Saos‐2) and human osteoblasts cell line (hFOB) was determined by qRT‐PCR. *P < .05 and **P < .01