Figure 1.

Cell viability and apoptosis detection of PDLSCs after knockdown of KDM6A. (A) Real‐time RT‐PCR confirming depleted expression of KDM6A in PDLSCs‐KDM6Ash compared with PDLSC‐WTs. (B) The cell viability of PDLSCs‐WT and PDLSCs‐KDM6Ash was determined by CCK‐8 assay everyday in 8 days (mean ± SD, n = 3). (C) The cell viability of PDLSCs‐WT and PDLSCs‐KDM6Ash after osteogenic or chondrogenic culture was determined at 3 days. (D) Flow cytometry analysis of PDLSCs‐WT or PDLSCs‐KDM6Ash with or without 3‐day chondrogenic culture. Flow cytometry was performed using a double‐staining method with FITC‐conjugated annexin V and PI