Figure 2.

Bioengineered tooth germs from single‐tooth germ cells in vitro culture. (A), Schematic representations of treatment of tooth germ cells for in vitro organ culture. The epithelium and mesenchyme of tooth germs were dissociated to single cells. The single cells were pelleted to reconstruct tooth germs with 3 ways and cultured in vitro for 8 d. (B), Ep‐Me in vitro culture and histological analysis (H&E staining, right panels), bioengineered tooth germs from re‐associated epithelial and mesenchymal single cells with compartment. (C), TG cells in vitro culture and histological analysis (H&E staining, right panels), bioengineered tooth germs from mixed tooth germ single cells. (D), Me in vitro culture and histological analysis (H&E staining, right panels), bioengineered tooth germs from re‐aggregated mesenchymal cells alone. Scale bar = 200 μm