Effects of cap on growth and distribution of DU145 cells. DU145 cells were treated with various concentrations of Capillarisin (Cap) for indicated time periods. (A), After treatment, cell growth was determined by WST‐1 assay. Cell growth of the vehicle‐treated group is set as 100% and data obtained from three independent experiments are expressed as the mean ± SE. (B), Protein expression of PARP and cleaved PARP after treatment with various concentrations of Cap as indicated for 72 hours was analysed by immunoblotting assays. (C), The fluorescence intensity for Annexin V‐FITC and PI in DU145 cells after treatment with or without Cap for 48 hours. (D), The cell cycle distribution after DU145 cells were treated with various concentrations of Cap as indicated for 24 hours