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. 2019 May 17;12:3765–3777. doi: 10.2147/OTT.S193675

Figure 3.

Figure 3

FOXP1 is a direct target gene of miR-605 in NSCLC cells. (A) Comparison of sequences of miR-605 with wild-type or mutant putative binding sites in the 3′-UTR of FOXP1 gene. (B) Luciferase reporter assay was employed to determine that miR-605 directly targets the 3ʹ-UTR of FOXP1. Luciferase activity was detected in H460 and H522 cells co-transfected with miR-605 mimics or miR-NC and wild-type pMIR-FOXP1-3ʹ-UTR or mutant pMIR-FOXP1-3ʹ-UTR. *P<0.05 vs miR-NC. (C, D) The FOXP1 mRNA and protein levels in NSCLC tissues and adjacent normal lung tissues were detected via RT-qPCR and western blot analysis, respectively. *P<0.05 vs normal lung tissues. (E) Spearman’s correlation analysis showed an inverse correlation between miR-605 and FOXP1 mRNA levels in NSCLC tissues. r=−0.5480, P<0.0001. (F, G) RT-qPCR and western blot analysis was adopted to measure the mRNA and protein levels of FOXP1 in H460 and H522 cells transfected with miR-605 mimics or miR-NC. *P<0.05 vs miR-NC.