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Expression of ESC‐markers in MMRC clones. ESC markers via immunofluorescence in B16F10 cells and MMRC clones. Reprogrammed cells are positive for ESC markers: Oct4 (A), Nanog (B) and Sox2 (C). (D) Secondary antibody negative control. (E) RT‐PCR analysis of ESC‐marker genes in B16F10 cell line, MMRC clones and mES positive control. Primers used for Oct3/4, Sox2, Klf4 and c‐Myc specifically detect the transcripts of the interest genes
