Table 1.

Primers details

Primer name	Sequence (5′ to 3′)	Sense	Fragment size (pb)	Tm (°C)
Oct4	CCTGGGCGTTCTCTTTGGAA	F	123	57.6
	GCTTCCTCCACCCACTTCTC	R		57.7
Nanog	TGGAAGCCACTAGGAAAGC	F	115	57.2
	GCCCAGATGTTGCGTAAGTC	R		56.3
Sox2	TTTGTCCGAGACCGAGAAGC	F	146	57.1
	CTCCGGGAAGCGTGTACTTA	R		56.4
β‐actin	GCTCCGGCATGTGCAAAG	F	114	59.8
	CCTTCTGACCCATTCCCACC	R		60.0