Figure 6.

YAP contributes to the activation of Akt‐mTOR by SAV1 knockdown. A, Western blot results showing SAV1 regulates the phosphorylation of YAP. DLD‐1 cells were infected with lentivirus expressing the indicated vectors for 24 h, and then the protein was harvested that subjected to western blot. B, Western blot results showing YAP is involved in the activation of the Akt‐mTOR signalling pathway by the SAV1 knockdown. DLD‐1 cells were infected with lentivirus expressing the indicated vectors for 24 h, and then the protein was harvested that subjected to western blot. C, Western blot results showing YAP phosphorylation level is reduced in colon cancer tissues. D, Quantitative results of phosphorylated YAP (p‐YAP) level in (C). The data were analysed by paired Student's t‐test. ***P<0.001. n=6 in each group