Table 1.
The primer sequences for analysed genes
| Target cDNA | Primer sequence (5′‐3′) | Product size (bp) | NCBI no. |
|---|---|---|---|
| GADPH | F CTTTGGTATCGTGGAAGGACTC | 132 | NM_002046.3 |
| R GTAGAGGCAGGGATGATGTTCT | |||
| ALP | F TAAGGACATCGCCTACCAGCTC | 170 | NM_000478.4 |
| R TCTTCCAGGTGTCAACGAGGT | |||
| DMP1 | F CTCGCACACACTCTCCCACTCAAA | 180 | NM_004407.3 |
| R TGGCTTTCCTCGCTCTGACTCTCT | |||
| DSPP | F GGAGCCACAAACAGAAGCA | 101 | NM_014208.3 |
| R TGGACAACAGCGACATCCT | |||
| Col‐IA1 | F AACATGGAGACTGGTGAGACCT | 145 | NM_000088.3 |
| R CGCCATACTCGAACTGGAATC | |||
| MEPE | F GGATGAAACTGCGAAAGAGG | 149 | NM_001291183.1 |
| R A CCTTCCCTTGGTGAGCATT | |||
| Sp7 | F GAGGTTCACTCGTTCGGATG | 120 | NM_001300837.1 |
| R TGGTGTTTGCTCAGGTGGT |
GADPH (glyceraldehyde‐3‐phosphatedehydrogenase), ALP (alkaline phosphatase), DMP1 (Dentin matrix phosphoprotein 1), DSPP (dentin sialophosphoprotein), Col‐IA1 (collagen‐IA1), MEPE (matrix extracellular phosphoglycoprotein), Sp7 (special protein 7).