Figure 1.

Polydimethylsiloxane (PDMS) substrates with varied stiffness induce osteoblasts to form distinct morphologies. (A) Schematic draft of osteoblasts exposed to PDMS substrates. (B) Stiff, intermediate and soft PDMS substrates fabricated by different proportions of base and curing agents (10:1, 30:1 and 50:1) displayed stiffness of 134, 16 and 1.4 kPa, respectively. The presented results are representative of three different samples (n=3). Data are presented as means ± SD. (C) SEM images of osteoblasts plated on varying elastic PDMS materials. Scale bars are 100 μm