Figure 2.

Rigid substrates promote osteoblastic differentiation. Macroscopic (A) and microscopic (B) images of alkaline phosphatase (ALP) staining in osteoblasts plated on stiff, intermediate and soft substrates after 14 days. Scale bars are 400 μm. (C) Transcriptional levels of Runx2 and ALP in osteoblasts at 7‐day post‐seeding. Data have been firstly normalized to GAPDH, and then normalized to that of group 10:1. The results shown are representative of three different samples (n=3). Data are presented as means ± SD, *P<.05, **P<.01