Table 1.
Sequences of forward and reverse primers of selected genes designed for qPCR
| mRNA | Product length | Primer pairs | |
|---|---|---|---|
| GAPDH | 233 bp | Forward | ACAGCAACAGGGTGGTGGAC |
| Reverse | TTTGAGGGTGCAGCGAACTT | ||
| Runx2 | 106 bp | Forward | CCTCTGACTTCTGCCTCTGG |
| Reverse | GATGAAATGCCTGGGAACTG | ||
| ALP | 101 bp | Forward | CCTGACTGACCCTTCCCTCT |
| Reverse | CAATCCTGCCTCCTTCCACT | ||
| Rac‐1 | 200 bp | Forward | GAGAGTACATCCCCACCGTC |
| Reverse | AACACGTCTGTTTGCGGGTA | ||
| RhoA | 132 bp | Forward | AACAGGATTGGCGCTTTTGG |
| Reverse | GATGAGGCACCCCGACTTTT | ||
| ROCK‐1 | 108 bp | Forward | TTGGTTGGGACGTACAGTAAAA |
| Reverse | CGTAAGGAAGGCACAAATGAGA | ||
| ROCK‐2 | 135 bp | Forward | GACATTGAACAGCTTCGGTCGGA |
| Reverse | CATTGAACAGCTTCGGTCG | ||