Figure 4.

The up‐regulation of ABCC1 is involved in visfatin‐induced non–small‐cell lung cancer (NSCLC) doxorubicin (Dox) resistance. (A) Cells were transfected with pcDNA3.1 (vector) or pcDNA/Visfatin for 24 hours, the expression of MRP1 and P‐gp was measured by western blot analysis; (B) A549 or H1793 cells were transfected with pcDNA3.1 (vector) or pcDNA/Visfatin for 24 hours, the mRNA expression of ABCC1 and ABCB1 was measured by qRT‐PCR; (C) A549/Dox and H1793/Dox cells were transfected with siRNA negative control (siNC) or si‐visfatin‐1 for 24 hours, the mRNA expression of ABCC1 and ABCB1 was measured by qRT‐PCR. *P<.05 compared with the control