Figure 1.

ELISA assay for CXCL12 in ADIPO CM and the effect of CXCL12 on the proliferation viability of RAW264.7 cells and BMMs. The adipogenic effects of ST2 cells under normal and differentiation conditions were measured by Oil Red O staining on day 21 (A, B). ELISA analysis for secreted CXCL12 concentration after adipocyte differentiation compared with α‐MEM culture (C). The proliferation effect of CXCL12 was evaluated using the CCK‐8 method. RAW264.7 cells and BMMs were cultured for 1, 2, 3 and 4 days in the presence or absence of varying concentrations of CXCL12. Optical density was measured at 450 nm. Cell proliferation viability was expressed as a percentage of the control (D, E). Values are mean ± SD of three independent experiments. **P<.01