Figure 4.

CXCL12 derived from adipocyte contributed to increase the expression of osteoclast functional specific molecules. RAW264.7 cells were cultured for 4 days with control conditions, with ADIPO CM or with ADIPO CM in the presence of recombinant CXCL12 or AMD3100. Quantitative RT‐PCR analysis of osteoclast specific genes: c‐src, cathepsin K and MMP‐9 in osteoclasts were performed (A) and data were graphed as fold change relative to control. Western blot of NFAT2, p‐src, MMP‐9 and cathepsin K in osteoclasts was performed, and band intensity analysis of respective protein levels in osteoclasts was normalized to β‐actin level and represented as % control. Values are mean ± SD of three independent experiments. Means with different letters differ significantly from each other P<.05