Figure 3.

The effect of adenosine on the mammosphere‐forming ability and cell viability of breast cancer stem cells (CSCs). The size of mammosphere formed upon treatment with adenosine was smaller than those in the medium without adenosine (A). Quantitative analysis of mammosphere formation (B). Adenosine reduced cell viability in breast CSCs and breast cancer cell lines (C). The effect of A3ARs antagonist on the adenosine‐induced cytotoxicity in breast CSCs (D). The results shown represent the mean ± SD of three independent experiments. *P<.05; **P<.01 compared with the control group