Figure 5.

miR‐1297 regulated NSCs proliferation and differentiation by regulating Hes1 expression. A, The mRNA expression of Hes1 was detected by using the qRT‐PCR method. B, The protein expression of Hes1 was measured by Western blot. C, The cell proliferation was determined by CCK‐8 assay. D, Representative images of neurospheres in different groups were shown. E, The relative neurosphere number was shown. F, The mRNA expression of β‐tubulin‐III was determined by qRT‐PCR. G, The protein expression of β‐tubulin‐III was measured by Western blot. H, The mRNA expression of GFAP was determined by qRT‐PCR. I, The protein expression of GFAP was measured by Western blot *p<0.05, **p<0.01 and ***p<0.001.