Figure 3.

The expression of USP32 is efficiently silenced in two small cell lung cancer (SCLC) cell lines. (A) USP32 expression in six SCLC cell lines using Western blot analysis; (B) statistical analysis of the ratio of USP32/ β‐actin; SCLC, small cell lung cancer; ***P<.001; β‐actin was used as an internal control. The mRNA and proteins expression of USP32 was significantly reduced when USP32 was silenced by specific siRNA targeting USP32 (SiUSP32) compared with untreated (Mock) and scrambled siRNA (NC) in GLC4 (C) and H1688 (D) cells. SCLC, small cell lung cancer; all the results are represented as mean ± SD from three independent experiments. ***P<.001 as compared with NC cells; β‐actin was used as an internal control