Figure 5.

USP32 silencing remarkably induced G0/G1 arrest in GLC4 cells. A, Representative images showed knockdown of USP32 altered cell cycle distribution profiles in GLC4 cells. B, The cell cycle was significantly arrested in G0/G1 phase in SiUSP32 group. C, Western blot analysis of proteins associated with cell cycle regulation in GLC4 cells after USP32 knockdown. D, The percentage of cells in sub‐G1 phase was significantly elevated in SiUSP32 group. Mock: untreated cells; NC: cells were treated with scrambled siRNA; SiUSP32: cells were treated with siRNA targeting USP32; all the results are represented as mean ± SD from three independent experiments. **P<.01, ***P<.001 as compared with NC cells; β‐actin was used as an internal control