Figure 6.

USP32 silencing remarkably promoted cell apoptosis, including early apoptosis and late apoptosis in GLC4 cells. A, GLC4 cells stained with Annexin V and 7‐AAD analysed using flow cytometry analysis. B, Quantification of the percentage of early apoptotic cells (Annexin V+/7‐AAD−) and late apoptotic cells (Annexin V+/7‐AAD+). C, Western blot analysis of proteins associated with cell apoptosis in GLC4 cells after USP32 knockdown. Mock: untreated cells; NC: cells were treated with scrambled siRNA; SiUSP32: cells were treated with siRNA targeting USP32; all the results were represented as mean ± SD from three independent experiments. ***P<.001 as compared with NC cells; β‐actin was used as an internal control