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. 2017 Oct 9;50(6):e12395. doi: 10.1111/cpr.12395

Figure 3.

Figure 3

LincRNA‐p21 down‐regulates pyruvate kinase M2 (PKM2) in a PTEN/AKT/mTOR cascade‐dependent manner. (A‐C) LincRNA‐p21 knockdown and control DU145 and LNCaP cells were subjected to qRT‐PCR analysis of lincRNA‐p21, and to Western blot with antibodies as indicated (B and C). (D) Cells described in (A) were used for qRT‐PCR analysis of PKM2. (E and F) LincRNA‐p21‐silenced DU145 cells were treated with LY294002 (LY) (E) or rapamycin (Rapa) (F) and then subjected to Western blot assay. (G‐I) LincRNA‐p21 overexpressed and control DU145 and LNCaP cells were subjected to qRT‐PCR analysis of lincRNA‐p21 (G), and to Western blot analysis of PKM2 (H and I). **P< .01; ***P< .001; ****P< .0001