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The reactive oxygen species (ROS) levels of 661W cells after exposure to different concentrations of zinc oxide (ZnO) NPs for 24 h assessed by flow cytometry. (A) Normal cells; (B) cells treated with 31.25 μmol/L of ZnO NPs; (C) cells treated with 62.5 μmol/L of ZnO NPs; (D) cells treated with 125.0 μmol/L of ZnO NPs; (E) result of histogram analysis. Data are from three independent experiments performed by flow cytometry. NPs=nanoparticles
