Figure 5.

Expressions of Na+/K+‐ATPase at mRNA and protein levels after treatment with different concentrations of zinc oxide (ZnO) nanoparticles. Cells were seeded in six‐well plates and cultured overnight, then treated with different concentrations (ie, 0, 31.25, 62.5, 125.0 μmol/L) of ZnO nanoparticles either for 2 h (for mRNA measurement) or for 6 h (for protein measurement). Next, alterations of Na+/K+‐ATPase in both mRNA (A) and protein (B) levels were determined using quantitative PCR and ELISA techniques respectively. Results were presented as mean ± SD (n=3)