Figure 7.

Effect of miRNA‐mediated TRPM1 knockdown on melanosome transfer. The MCs were transfected with TRPM1 siRNA and the scramble control for 48 h. Efficiency of TRPM1 knockdown by these siRNAs was tested using Western blotting and RT‐PCR respectively (A). The histogram (B) shows the densitometric quantification of data with means ± SEM of three independent experiments. TRPM1 siRNA‐transfected MCs were co‐cultured with KCs as described in Figure 1. The co‐cultured cells were further exposed to 3 J/cm² UVA and 20 mJ/cm² UVB. The cells were harvested at the indicated time after UV radiation for flow cytometry analysis. Representative fluorescence dot‐plots from flow cytometry analyses are shown in (C). The histogram (D) shows the means ± SEM of fluorescence intensities from three independent experiments. *P < .05, ^# P < .01