Figure 5.

Knockdown of GBAS in CAL-27 cells inhibited tumorigenicity in nude mice. sh-GBAS or sh-Ctrl transfected cells were transplanted into inoculated subcutaneously into nude mice for up to 26 days (n = 10).

Note: (A) After scarification, xenograft tumors in vivo and excised tumors were recorded. (B) Tumor volume was estimated based on the following equation: volume =1/2 × (largest diameter) × (smallest diameter)2 and data were shown as mean ± SD (n = 10 in each group), **P<0.01. (C) Tumor weight was obtained at the end of experiment and was displayed as mean ± SD (n = 10 in each group), **P<0.01. (D) Tumor growth was systemically administered and monitored by FI. At the end of the experiments, tumor tissues were exposed to FI. Images represent the radiant efficiency. (E) Regions of interest calculated for the tumor and results were expressed as total radiant efficiency ([p/s]/[μW/cm2]), **P<0.01.

Abbreviations: GBAS, glioblastoma amplified sequence; NC, normal control; KD, knockdown; Avg, average; FI, fluorescence imaging.