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. Author manuscript; available in PMC: 2019 May 21.
Published in final edited form as: J Mol Biochem. 2018;7:48–59.

Figure 5.

Figure 5.

Flow-induced changes in cAMP levels and COX-2 expression in osteocytes overexpressing intact and mutated AC6. MLO-Y4s were transfected with AC6 OE, AC6 Mut OE, or Control OE plasmids and exposed to 2 or 30 min OFF. After 2 min of OFF, the AC6 Mut OE group exhibits an increase in cAMP production (A) and decreased osteogenic response (C) compared to the AC6 OE group. There is no difference in cAMP production (B) or COX-2 expression (D) between the groups with 30 min of OFF. Bar graphs represented fold changes with OFF compared to static controls. AC6 OE and AC6 Mut OE are normalized to Control OE. Data are presented as mean ± SEM. n = 6 for all groups, *p < 0.01. These experiments were repeated 3 times for each group.