Table 1.
Mutation and sequencing primers for mutation plasmid design
| Plasmid | Mutation Primers | Sequencing Primer |
|---|---|---|
| AC3 Mut OE | 324 For: TCCTCTTTGCCGCCATCG 324 Rev: TGCTGACGTTCTCGTGACG 368 For: TTAAGATCCTGGGCGCCTGC 368 Rev: TCCGCAGCTGGTGGTATTTAGC |
For: TCTATGACGGTGATGGTGTTCC |
| AC6 Mut OE | 382 For: TTGAGGGCTTCACCAGCCTG 382 Rev: TGGCCGCAAACAGGATGCTG 426 For: TAGGAGCCTGTTACTACTGC 426 Rev: AGATCTTGATCCTCAGACAG |
For: TGTCTGCACACACTACCCTG |
| TRPV4 Mut OE | For: CTTCTCCCTGAACAAGAACTCAAGC Rev: TTGTTCAGGGAGAAGCCATAGTACTG |
For: TCTCTACCACTGACACCCGTTC |