Array of projections of the most specific split-GAL4 line for each cholinergic LHON cell-type. Where available, a neuron from that class traced to at least to identification in the EM volume (inset, black represents tracing, grey the lateral horn, blue and brown the single neuron labelling, Nern et al., 2015). Expression of split-GAL4 lines are visualized using UAS-csChrimson::mVenus in attP18 (green), with nc82 as a neuropil stain (magenta). Each image is registered to JFRC2010, while the EM data is registered to JFRC2013. The cell-type (eg. PV5a1) labelled is in the top left of each panel, while the line code (eg. LH290) is listed below. Cell-types that stained positive for more than one neurotransmitter are labelled with an asterix in the top right. Off-target expression in the brain for non-ideal lines labelled with a yellow arrow. See www.janelia.org/split-gal4 for image data.