Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
Drosophila split-GAL4 Line, LH989 |
Dolan et al., 2018 | LH989 |
http://splitgal4.
janelia.org/cgi-bin/splitgal4.cgi |
| Genetic reagent (D. melanogaster) |
Drosophila split-GAL4 Line, LH991 |
Dolan et al., 2018 | LH991 | http://splitgal4.janelia.org/cgi-bin/splitgal4.cgi |
| Genetic reagent (D. melanogaster) |
Drosophila split-GAL4 Line, LH2447/MB077B |
Aso et al., 2014a | LH2447/MB077B | http://splitgal4.janelia.org/cgi-bin/splitgal4.cgi |
| Genetic reagent (D. melanogaster) |
Drosophila split-GAL4 Line, LH2449/MB380B |
Aso et al., 2014a | LH2449/MB380B | http://splitgal4.janelia.org/cgi-bin/splitgal4.cgi |
| Genetic reagent (D. melanogaster) |
Drosophila LH split-GAL4 Line, LH split-GAL4 lines generated in this study |
this study, see Supplementary file 1 | LHXXXX | http://splitgal4.janelia.org/cgi-bin/splitgal4.cgi |
| Genetic reagent (D. melanogaster) |
csChrimson Reporter/Optogenetic effector, 20xUAS- csChrimson::mVenus (attP18) |
Klapoetke et al., 2014 | 55134, Bloomington Stock Center |
|
| Genetic reagent (D. melanogaster) |
Polarity reporter , w; +; 3xUAS-Syt:: smGFP-HA (su(Hw)attP1), 5xUAS-IVS- myr::smGFP-FLAG (VK5) |
Aso et al., 2014a | ||
| Antibody | Mouse anti-Brp, monoclonal |
DSHB, University of Iowa, USA |
nc82 | 1/30 |
| Antibody | Rabbit anti-GFP, polyclonal |
Life Technologies | A-11122 | 1/1000 |
| Antibody | Goat anti-Rabbit AF488 |
Life Technologies | A-11008 | 1/400 |
| Antibody | Goat anti-Rabbit AF568 |
Life Technologies | A-11004 | 1/800 |
| Antibody | Rat anti-FLAG, monoclonal |
Novus Biologicals | NBP1-06712SS | 1/200 |
| Antibody | Rabbit anti-HA, monoclonal |
Cell Signalling Technologies |
C29F4 | 1/300 |
| Antibody | ATTO 647N Goat α-Rat IgG |
Rockland | 612-156-120 | 1/300 |
| Antibody | Cy3 Goat α-Rabbit |
Jackson Immuno Research |
111-165-144 | 1/1000 |
| Antibody | Cy2 Goat α-Mouse |
Jackson Immuno Research |
115-225-146 | 1/600 |
| Antibody | Mouse α-V5, polyclonal |
AbD Serotec | DL550 | 1/500 |
| Antibody | AF488 Goat α-Mouse |
Life Technologies |
A-11001 | 1/400 |
| Antibody | AF594 Donkey α-Rabbit |
Jackson Immuno Research |
A-21207 | 1/500 |
| Chemical compound, drug |
Paraformaldehyde | Electron Microscopy Sciences |
#15713 | |
| Software, algorithm |
Fluorender | Wan, 2012 | http://www.sci.utah.edu/software/fluorender.html | |
| Software, algorithm |
Fiji | Schindelin et al., 2012 | http://fiji.sc/ | |
| Software, algorithm |
CMTK | https://www.nitrc.org/projects/cmtk/ | ||
| Software, algorithm |
CATMAID | Schneider-Mizell et al., 2016 | www.catmaid.org | |
| Software, algorithm |
R | https://www.r-project.org/ | ||
| Software, algorithm |
nat | 10.5281/zenodo.1136106 | http://jefferis.github.io/nat/ | |
| Software, algorithm |
elmr | Zheng et al., 2018 | 10.5281/zenodo.1401050 | https://jefferis.github.io/elmr/ |
| Software, algorithm |
lhns | This study and Frechter et al., 2019 |
10.5281/zenodo.2631765 | https://github.com/jefferislab/lhns |
| Software, algorithm |
2018lhsplitcode | This study |
https://github.com/jefferislab/2018lhsplitcode | |
| Software, algorithm |
flywatch | This study | 10.5281/zenodo.2631286 | https://github.com/jefferislab/flywatch |
| Software, algorithm |
LHLibrary | Frechter et al., 2019 | 10.5281/zenodo.2635574 | https://github.com/jefferislab/LHlibrary |
| Software, algorithm |
NMF | Gaujoux and Seoighe, 2010 | https://cran.r-project.org/package=NMF |