Figure 4. T follicular helper cells are generated by RSV M-null infection.
BALB/c mice were infected with either RSV recWT or RSV M-null, and mLN and lungs were harvested on days 14 and 21 p.i. Cells were gated on CD4 T cells, and gates were placed using fluorescence minus one controls. Representative staining panels of Bcl6+CXCR5+ Tfh cells in the (A) mLN and (C) lung. Total numbers of Bcl6+CXCR5+ Tfh cells in the (B) mLN and (D) lung. Data are presented as mean ± SEM of 2 independent experiments (n=8). Groups were compared using Student’s t test.
