FIGURE 2.

Seeding of kidney progenitors in silk scaffold. Experiments were performed in triplicate and representative examples are shown. (A) Schematic illustration of spin-seeding in silk scaffold (0.2mm thick and 2mm diameter) by centrifugation at 300g for 6min. (B) Representative fluorescent image showing DiI labeled cells packed (dotted line) into the pockets of silk scaffold (white arrow) after spin-seeding. The DAPI channel was included in this image to show the localization of the autofluorescent silk, although DAPI staining was not performed. (C) Phalloidin 488 staining 3 days after seeding showing maintenance of cells packed into the pockets of silk scaffold. DAPI counterstain shows nuclei and the silk scaffold (arrows).