Effects of low O2 on IHMGEC proliferation and differentiation. Low O2 treatment did not impact the cell growth (A) or the expression of PCNA (B). SREBP-1, but not Lamp-1, expression is significantly increased in a hypoxic environment (C and D, † p < 0.05, n = 3 experiments). AZM with or without low O2 significantly increased the levels of Lamp-1 protein. (E, * p < 0.05, ** p< 0.01, n=3 experiments). DM = Differentiation Medium (DMEM/F12 + 10% FBS). PM = Proliferation Medium (KSFM + EGF/BPE).