Influence of low O2 on lipid and lysosome accumulation in IHMGECs. IHMGECs were treated were cultured in normoxic (21% O2) and hypoxic (3% O2) conditions environments in the presence or absence of AZM and then stained for neutral lipids (LipidTox Neutral Green) and lysosomes (LysoTracker blue). (* p < 0.05, ** p < 0.01, *** p< 0.001, **** p < 0.0001, one-way ANOVA, n = 3 experiments). Inserts show a section of the LysoTracker staining in black and white. Scale bar = 50 μM.