Impact of low O2 on DNase II activity and cell number in IHMGECs under differentiating conditions. DNase II activity is significantly increased by low O2 treatment in both cell lysate (A) and supernatant (B). Low O2 also significantly decreased the cell number under differentiating condition (C). DM = Differentiation Medium (DMEM/F12 + 10% FBS). (* p < 0.05, *** p< 0.001, **** p < 0.0001, one-way ANOVA. f p < 0.05, †† p < 0.01, two-tailed t-test. n = 3 experiments)