KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Sodium Chloride	Fisher BioReagents	BP358–212
Peptone	Fisher BioReagents	BP1420–2
Yeast Extract	Fisher BioReagents	BP1422–500
Agar	Fisher BioReagents	BP1423–500
Yeast nitrogen base without amino acids and ammonium sulfate	Sigma	Y1251–1KG
D-Glucose	Fisher Chemical	D16–3
Ammonium Sulfate	Fisher BioReagents	BP212R-1
Lithium Chloride	Growcells	MRGF-1336
Copper sulfate	Sigma	C1297–100G
Lactose Monohydrate	Fisher Chemical	L5–500
D-(+)-Xylose	Sigma	X1500–500G
Critical Commercial Assays
RNeasy Mini Kit	QIAGEN	74106
MaterPure Yeast DNA Purification Kit	Lucigen	MPY80200
SuperScript III First-Strand Synthesis System	Invitrogen	18080051
Power SYBR Green PCR Master Mix	Applied Biosystems	4367659
Q5 High-Fidelity 2X Mater Mix	New England BioLabs	M0492L
Nextera DNA Flex Library Prep	Illumina	20018705
Nextera DNA CD Indexes	Illumina	20018708
Deposited Data
List of all mutations identified in this study	This paper	Data S1
Experimental Models: Organisms/Strains
S. cerevisiae: BY4743	National Collection of Yeast Cultures	https://www.ncyc.co.uk/
Oligonucleotides
PCR primers used for mutation verification	This paper	Table S3
Software and Algorithms
Burrows-Wheeler Aligner	[45]	http://bio-bwa.sourceforge.net/bwa.shtml
Genome Analysis Toolkit	[46]	https://software.broadinstitute.org/gatk/