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. 2019 Mar 27;33(6):7490–7504. doi: 10.1096/fj.201802391R

Figure 6.

Figure 6

APP cleavage by the meprin heterodimer. A) Diagram of APP cleavage by cell surface–bound meprin β and impaired cleavage by soluble meprins. Cleavage of cell surface–bound meprin β and subsequent γ-secretase cleavage leads to generation of accumulating Aβ fragments in the extracellular space. Catalytically inactive meprin β E153A was used for the meprin enzyme complex to exclude meprin β cleavage. B) Transfection of HeLa cells with APP, meprin WT, or meprin β E153A variant. Immunoblotting of cell lysates and supernatants revealed cleavage of APP and Aβ generation by active meprin β, not by membrane-bound meprin α or inactive meprin β. Recombinant Aβ served as positive control. Mep, meprin; N-, N terminus; rec., recombinant.