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. 2019 Mar 18;33(6):7451–7466. doi: 10.1096/fj.201802338RR

Figure 5.

Figure 5

Overexpression of Tsg101 enhances recycling of IGF-1R in cardiomyocytes. A, B) Representative immunoblots (A) and quantitative analyses (B) of streptavidin immunoprecipitates (IP), visualized using an IGF-1R antibody. Whole-cell lysates (WCLs) were used as loading controls. Four independent experiments were performed for recycling assays. C) Representative images of immunofluorescence staining with streptavidin Alexa Fluor 594 conjugate after IGF-1R biotin was internalized and recycled in Ad.Tsg101 and Ad.GFP NRCMs; n = 4 plates, 25–30 cells per plate. Scale bars, 10 μm. *P < 0.001.