Figure 1.
NMN induces HO-1 and SRXN1 expression in astrocytes. A) Primary confluent spinal cord astrocyte cultures obtained from nontransgenic (NonTG) or hSOD1G93A (G93A) mice were treated with vehicle (control) or 5 mM NMN. Twenty-four hours later, Hmox1, Srxn1, and Nrf2 mRNA levels were determined by real-time PCR and corrected by actin mRNA levels. B) Western blot analysis of HO-1 protein levels in NonTG and G93A astrocytes following 24 h of treatment with 5 mM NMN. C) Quantification of HO-1 expression after correction by actin levels. D) Western blot analysis of SRXN1 protein levels in NonTG and G93A astrocytes following 24 h of treatment with 5 mM NMN. E) Quantification of SRXN1 expression after correction by actin levels. For all graph panels, each data bar represents the mean ± sd of at least 3 independent experiments. *P < 0.05, significantly different from vehicle (control)-treated NonTG astrocytes.