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. 2019 Mar 20;33(6):7778–7790. doi: 10.1096/fj.201802799R

Figure 3.

Figure 3

The PDK4 activator WY-14643 determines myotube atrophy. A) Assessment of myotube size in C2C12 cultures treated with WY-14643 for up to 48 h. On average, 250–350 myotubes were measured per experimental condition in triplicate. Green staining: myosin heavy chain. Scale bar, 100 μm. B) Representative Western blotting for PDK4 in whole protein extracts from C2C12 treated with WY-14643 (n = 3). Tubulin was used as loading control. Data (means ± sd) are expressed as fold change vs. control. *P < 0.05, ***P < 0.001.