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. 2019 Feb 28;33(6):6919–6932. doi: 10.1096/fj.201802433R

Figure 2.

Figure 2

Mif−/− mice show increased pulmonary neutrophil accumulation, greater cytokine production, and lower survival after TSST-1 injection. WT or Mif−/− BALB/c mice were injected intraperitoneally with 1 mg/kg of TSST-1. A) BAL and lung cells were prepared at 72 h postinjection, and neutrophil recruitment was examined by flow cytometry. B, C) Levels of the neutrophil chemoattractant MIP-2 in the lungs (B), and cytokine levels in BAL determined by ELISA (C). Data are representative of 2 replicates. *P < 0.05, **P < 0.01, by unpaired Student’s t test. D) WT BALB/c or Mif−/− mice were injected intraperitoneally with 1 mg/kg of TSST-1 and 0.9 mg/kg of d-galactosamine and monitored for survival. PerCP, peridinin-chlorophyll protein. Data are representative of 2 experiments with n = 10 mice per group. P = 0.0062 (Gehan-Breslow-Wilcoxon Test).