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. 2019 Feb 28;33(6):6919–6932. doi: 10.1096/fj.201802433R

Figure 3.

Figure 3

IL-1β and IL-23 promote IL-17A production from γδ T cells in the absence of TCR engagement. A) Spleen cells from WT or Mif−/− BALB/c mice were stimulated with 20 ng/ml of IL-1β, 20 ng/ml of IL-23, or both for 72 h and stained for intracellular IL-17A and for surface CD3, CD4, and γδ TCRs. Numbers refer to percentage of CD3+CD4+IL-17A+ cells or CD3+γδTCR+IL-17A+ cells. B) LM induces IL-1β and IL-23 production by macrophages. BMDMs from WT or Mif−/− BALB/c mice were stimulated with 1 µg/ml of LM for 18 h. IL-1β and IL-23 concentrations in the supernatants were quantified by ELISA. Comp, compensation; ctrl, control; FL2-H, flourescence 2 channel height; Gr, granulocyte. Data shown are representative of 3 replicates with n = 4–5 per group. *P < 0.05, **P < 0.01 (unpaired Student’s t test).