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. 2019 Feb 28;33(6):6919–6932. doi: 10.1096/fj.201802433R

Figure 7.

Figure 7

γδ T-cell depletion increases survival and reduces Mif-dependent IL-17 production after TSST-1 administration. A) WT or γδ−/− BALB/c mice were injected intraperitoneally with TSST-1 and d-galactosamine as described in Materials and Methods and survival monitored (n = 18 mice). B) For antibody-mediated γδ T-cell depletion, mice were injected intraperitoneally with anti–γδ TCR mAb (UC7-13D5) or control isotypic Ab (200 μg) on d −3 (n = 20 mice; Gehan-Breslow-Wilcoxon test). Lung cells were prepared at 72 h postinjection of TSST-1/d-galactosamine. C, D) Number of CD3+ γδ TCR+ cells and IL-17A–producing γδ T cells was enumerated by flow cytometry (C), and concentrations of IL-17A and MIP-2 were determined in BAL by ELISA (D). Ctrl, control. Data are representative from 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired Student’s t test).