Skip to main content
. 2019 May 21;10(6):395. doi: 10.1038/s41419-019-1627-9

Fig. 5. KPT-330 inhibits rRNA processing.

Fig. 5

a Quantification of RNA content per cell in U87, U251, and H1299 cells treated with KPT-330 (1 μM) for 24 h. (mean ± SEM, n = 6). *P < 0.05. b Real-time PCR analysis of indicated rRNAs in U87, U251, and H1299 cells treated as in a (mean ± SEM, n = 6 for U87 and U251 and n = 8 for H1299). *P < 0.05. c Nucleolar RNAs from XPO1 (C528S) expressing U87, U251, and H1299 cells treated with KPT-330 (1 μM) for 24 h or Act D (500 ng/μl) for 3 h were subjected to fragment analysis. Quantification of the relative percentage of 18S, 28S, 32/34S, and 45/47S was shown in d–g (mean ± SEM, n = 3–6), respectively. *P < 0.05. h Nascent RNA synthesis in U87, U251, and H1299 cells treated as in a was measured by EU incorporation (mean ± SEM, n = 5). *P < 0.05. i Real-time PCR analysis of cytosolic and nuclear rRNAs indicated in U87, U251, and H1299 cells treated as in a (mean ± SEM, n = 4 for U87 and n = 5 for U251 and H1299). *P < 0.05