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. 2019 May 21;10:2261. doi: 10.1038/s41467-019-08620-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Establishment of a high-throughput screening assay for identification of small-molecule h-cGAS inhibitors. a Schematic showing dsDNA-activated cGAS synthesis of cyclic GMP–AMP (cGAMP) from ATP and GTP. b, c Assessment of enzymatic activity of h-cGAS under varying dsDNA concentrations based on RapidFire mass spectrometry (RF-MS) assay (b) or ATP-coupled luminescence (LUM)-based assay (c). d Schematic for high-throughput screening of small-molecule h-cGAS inhibitors using assay (c). Numbers in parentheses indicate the column(s) in plate into which the specified reagent was dispensed. RT room temperature. (n = 6 for b and c; mean ± S.D.)