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. 2019 May 21;9:7672. doi: 10.1038/s41598-019-43964-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Restoration of correct β-globin mRNA splicing and HbA production by U7.BP + 623 snRNA lentiviral vector in erythroid cells. Correction of β-globin mRNA splicing and HbA production in β^IVS2-654-thalassaemia/HbE erythroid progenitor cells transduced with U7.BP + 623 snRNA lentiviral vector. (A,B) correction of β-globin mRNA splicing was analysed by semi-quantitative RT-PCR labelled with Cy5-dCTP. (C,D) HbA production was analysed by HPLC. Haemoglobin of normal subject was used as control (normal); (E) Correlation analysis of correctly spliced β-globin mRNA and HbA. Correlation analysis was performed using Spearman Rank method (p < 0.05). UT, untransduced cells; Mock, mock control; SCR, scramble control; U7.BP + 623, U7.BP + 623 snRNA lentiviral vector; HbF, fetal haemoglobin; HbA, adult haemoglobin; HbE, haemoglobin E. Arrows indicate restored HbA.